RNAi: Mechanism of Gene Silencing and Applications - Concept | Molecular Biology | JoVe

SOLVED: RNA interference (RNAi) is a mechanism of gene silencing that is mediated by the presence of double-stranded RNA. Arrange the steps involved in gene silencing by RNAi:1. Double-stranded RNA (dsRNA) introduced into cell.2. Gene expression silenced.3. mRNA degraded.4. RNA-induced silencing complex (RISC) binds to the target RNA.5. Target RNA cleaved by RISC.6. Gene silencing achieved.

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