Recent advances in membrane mimetics for membrane protein …
The most established membrane mimetics are based on either proteins (nanodiscs and Salipro), short peptides (peptidiscs), or synthetic polymers (SMALPs). With different mimetics available, how do researchers decide which one is most suitable for their research question?
Advances in membrane mimetics and mass spectrometry for …
Aug 1, 2022 · This review presents recent developments in membrane mimetics and complementary mass spectrometry approaches for the investigation of membrane protein–lipid interactions after protein expression and purification.
Advances in Membrane Mimetic Systems for Manipulation and …
Feb 5, 2024 · Here we review advances in the development of membrane mimetic systems and give a range of examples of the membrane protein structures that have been obtained using them.
From bottom-up to cell surface proteomics: detergents or no …
Looking ahead, membrane mimetics show promise in overcoming challenges associated with detergents in proteomics. Unlike FASP, SP3, SP4 and S-Trap, membrane mimetics offer the advantage of swiftly removing detergents upon solubilization while preserving MPs in …
Lipid Membrane Mimetics in Functional and Structural Studies of …
Sep 3, 2021 · Here, we review the most widely used membrane mimetics in structural and functional studies of IMPs. These membrane mimetics are detergents, liposomes, bicelles, nanodiscs/Lipodisqs, amphipols, and lipidic cubic phases.
The importance of the membrane for biophysical measurements
Nov 16, 2020 · Here we consider the co-development of membrane mimetics with technological breakthroughs in both cryo-electron microscopy (cryo-EM) and native mass spectrometry (nMS).
A 10-year meta-analysis of membrane protein structural biology ...
Mar 1, 2021 · Here we analyze detergents and membrane mimetics (a.k.a. non-detergent alternatives) used for the solubilization and structure determination of membrane proteins, and draw correlations between these choices and the structure determining method of choice, protein taxonomic domain (i.e. prokaryotic or eukaryotic), and protein classification (i.e ...
Recent advances in membrane mimetics for membrane protein …
May 12, 2023 · Membrane mimetics are useful tools for stabilizing membrane proteins outside their native environment in a water-soluble format that is compatible with downstream structural and biochemical analysis. Summary of current thinking: Several membrane mimetic systems have been developed in the last two decades.
Fake It ‘Till You Make It—The Pursuit of Suitable Membrane Mimetics …
Dec 23, 2020 · In this review, we survey the currently available membrane mimetic environments ranging from detergent micelles to bicelles, nanodiscs, lipidic-cubic phase (LCP), liposomes, and polymersomes. We discuss their respective advantages and disadvantages as well as their suitability for downstream biophysical and structural characterization.
Lipid Membrane Mimetics in Functional and Structural Studies …
Sep 3, 2021 · Here, we review the most widely used membrane mimetics in structural and functional studies of IMPs. These membrane mimetics are detergents, liposomes, bicelles, nanodiscs/Lipodisqs, amphipols, and lipidic cubic phases.