Deterministic early endosomal maturations emerge from a …
Aug 2, 2023 · We show that very early endosome (VEE) to early endosome (EE) conversion is a multistep process, underpinned by the multiple asymmetric binding sites of EEA1 and its cyclical conformation...
Endoplasmic reticulum–endosome contact increases as endosomes …
Apr 1, 2013 · Here we use high-resolution EM to find that both the ER and endosomes contact microtubules (MTs) near their membrane contact sites, and this is likely to facilitate dynamic coupling between the ER and endosomes during trafficking.
The Complex Ultrastructure of the Endolysosomal System - PMC
Today the CURL is known as the “early endosome” (EE), which in addition to receptors and ligands is now known to be reached by virtually all components internalized from the cell surface (see Mayor et al. 2014; Cossart and Helenius 2014).
Tracking down the elusive early endosome - ScienceDirect
Nov 1, 2007 · Receptors are usually separated from their ligands in the early endosome (EE) and are recycled back to the PM via clathrin-coated vesicles whereas EEs mature into the late endosome (LE). Endosomal compartments in mammalian cells characteristically have internal vesicles and are often called ‘multivesicular endosomes’ 29 , 30 .
Functional cycle of EEA1-positive early endosome: Direct ... - PLOS
May 1, 2020 · Early endosomes, regarded as the main sorting station on endocytic pathway, are characterized by high frequency of homotypic fusions mediated by tethering protein EEA1. Despite intensive investigations, biogenesis of endosomes, boundaries between early and late endosomes, and process of cargo transition though them remain obscure.
Early Endosome Morphology in Health and Disease - PMC
Early endosomes are responsible for nutrient uptake, degradation of metabolic by-products, transport of materials to specific compartments in the cell, and regulating the cell-surface expression of receptors and transporters.
Tracking down the elusive early endosome - Cell Press
However, recent data indicate that the trans-Golgi network serves as an early endosome merging the secretory and endocytic pathways in plant cells.
Quantitative correlative microscopy reveals the ultrastructural ...
Jan 1, 2022 · The key endosomal regulators Rab5, EEA1, and APPL1 are frequently applied in fluorescence microscopy to mark early endosomes, whereas Rab7 is used as a marker for late endosomes and lysosomes. However, endogenous levels of these proteins localize poorly in immuno-EM, and systematic studies on their native ultrastructural distributions are lacking.
Early Endosome Capture Proteomics and its Application to …
Mar 31, 2022 · Here, we develop an approach for rapid isolation of early/sorting endosomes through affinity capture of the early endosome-associated protein EEA1 (Endo-IP) and provide proteomic and lipidomic snapshots of early endosomes in action.
Endosome maturation - The EMBO Journal
The maturation programme entails a dramatic transformation of these dynamic organelles disconnecting them functionally and spatially from early endosomes and preparing them for their unidirectional role as a feeder pathway to lysosomes.