Whole-cell 3D STORM reveals interactions between cellular structures ...
Nov 23, 2008 · Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions.
Three-Dimensional Super-Resolution Imaging by Stochastic …
Three-dimensional (3D) nanoscale-resolution imaging, however, remains a challenge. We demonstrated 3D stochastic optical reconstruction microscopy (STORM) by using optical astigmatism to determine both axial and lateral positions of …
Zhuang Research Lab - Harvard University
May 3, 2024 · Comparison of conventional (upper) and 3D STORM (lower) images of actin in the axons of neurons. Actin is labeled with phalloidin conjugated to photoswitchable dyes. STORM image revealed a novel periodic, actin-spectrin-based membrane skeleton in axons.
Automated highly multiplexed super-resolution imaging of ... - Nature
Mar 25, 2020 · Here, we report maS 3 TORM (multiplexed automated serial staining stochastic optical reconstruction microscopy), a microscopy approach capable of fully automated 3D direct STORM (d STORM)...
Whole-cell 3D STORM reveals interactions between cellular ... - PubMed
Here we demonstrated multicolor three-dimensional (3D) stochastic optical reconstruction microscopy (STORM) as a tool to quantitatively probe cellular structures and their interactions.
Rapid 3D-STORM imaging of diverse molecular targets in tissue
Jul 18, 2022 · We show that RAIN-STORM imaging is versatile and provide 3D nanoscopic data for over 20 synapse, neuron, glia, and vasculature targets. Sample preparation is also rapid, with a 1-day turnaround from tissue to image, and parameters are suitable for multiple tissue sources.
Stochastic Optical Reconstruction Microscopy (STORM): A …
The article discusses photoswitchable fluorescent molecules, STORM microscope design and the imaging procedure, data analysis, imaging of cultured cells, multicolor STORM, and three-dimensional (3D) STORM.
Nano-imaging with STORM - Nature Photonics
Aug 1, 2009 · Multicolour, three-dimensional stochastic optical reconstruction microscopy now makes it possible to image cellular structures with near molecular-scale resolution.
Whole Cell 3D Super-Resolution Imaging by STORM - Andor
A novel 3D super-resolution microscopic technique has made it possible to gain new insights into previously unresolvable environments. The approach of 3D Stochastic Optical Reconstruction Microscopy (‘STORM’) described by the Zhuang lab, has recently enabled the team to image whole cells in multi-color.
Rapid 3D-STORM imaging of diverse molecular targets in tissue
Jul 18, 2022 · We show that RAIN-STORM imaging is versatile and provide 3D nanoscopic data for over 20 synapse, neuron, glia, and vasculature targets. Sample preparation is also rapid, with a 1-day turnaround from tissue to image, and parameters are suitable for multiple tissue sources.