Antibody Discovery and B Cell Profiling Using Single ... - 10x Genomics
Nov 11, 2019 · The method, LIBRA-seq, uses the 10x Genomics Chromium Single Cell Immune Profiling Solution to recover DNA-barcoded antigens and B-cell receptor sequences simultaneously, overcoming the limitations of fluorescence based screening methods which can only mark a select number of antigens at a time.
Massively parallel single-cell B-cell receptor sequencing enables …
Aug 9, 2019 · Here we use high-throughput single-cell B-cell receptor sequencing (scBCR-seq) to obtain accurately paired full-length variable regions in a massively parallel fashion. We sequenced...
Single cell RNA-seq: An introductory overview and tools ... - 10x Genomics
10x Genomics’ single cell RNA-seq (scRNA-seq) technology, the Chromium Single Cell 3’ solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.
Generation of a single-cell B cell atlas of antibody repertoires and ...
We isolated bone marrow plasma cells (BM PCs) (CD138 hi, TACI hi) and spleen B cells (CD19 hi, IgM low, IgD low) by fluorescence-activated cell sorting (FACS) and performed single-cell antibody repertoire and transcriptome sequencing using the 10x genomics 5′ immune profiling pipeline (Figure 1A). Following library preparation, deep ...
Can I study T and/or B cell immune repertoires, gene ... - 10X Genomics
The Single Cell Immune Profiling Solution offers the option to generate: an enriched T cell library and/or an enriched B cell library, and/or a 5’ gene expression library, as well as a Cell Surface Protein library from the same cells.
Single Cell Immune Profiling - 10x Genomics
What options are available for antigen specificity studies with the Chromium Single Cell Immune Profiling assay? Can I titrate the ratio of biotinylated antigen to BEAM Conjugate to improve the detection of antigen-positive cells in a BEAM-Ab experiment?
Universal 5' Gene Expression - 10x Genomics
Alternative transcript isoform detection with single cell and spatial resolution
Consult the user guide for a consumables and equipment list validated by 10x Genomics for executing the Chromium Single Cell 3’ Gene Expression protocol. To enable seamless experimental planning, a breakdown of the protocol steps, along with execution times and stop & store points are listed below.
associate individual reads back to the individual partitions. This document outlines the protocol to generate an enriched T-cell library and/or an enriched B-cell library, and/or a 5’ G. with cells, and Partitioning Oil on Chromium Next GEM Chip G. To achieve single cell resolution, cel.
10X Genomics
Chromatin accessibility and transcriptional regulation at the single-cell level. An analysis platform to simplify and accelerate the interpretations of 10x data. Subcellular profiling of targeted features from fresh frozen or FFPE tissues.
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