Microplate Selection and Recommended Practices in High …
Jun 1, 2020 · High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The choice of microplate is a crucial yet often overlooked technical decision in HTS and quantitative biology.
Bacterial Growth Curves in 96-well microplates - protocols
Starting cell density for plate reader experiments: Growth curves should be performed in the dynamic range of the instrument (e.g. at time 0, wells with bacteria should have net positive A600 readings after blank subtraction).
High-throughput cultivation and identification of bacteria …
Jan 13, 2021 · First, to streamline the tedious process of colony picking and repeatedly obtaining fast-growing bacteria, we isolated the bacteria in 96-well cell culture plates by performing a limiting...
Precise, High-throughput Analysis of Bacterial Growth - PMC
Correctly analyzing bacterial growth is crucial for understanding the growth-dependent coordination of gene functions and cellular components. Accordingly, the precise quantitative evaluation of bacterial growth in a high-throughput manner is required.
Bacterial Growth Curve Measurements with a Multimode …
Recently, we developed a new analysis method for evaluating bacterial growth with microplate readers based on time derivatives. This method not only uses the optical density (i.e., absorption/scattering) of bacterial culture, but also takes advantage of the fluorescence of bacteria expressing fluorescent proteins (Krishnamurthi et al., 2021 ).
A method for high throughput determination of viable bacteria …
Nov 13, 2012 · Here, we report a bacterial cell counting method termed Start Growth Time (SGT) that allows rapid and serial quantification of the absolute or relative number of live cells in a bacterial culture in a high throughput manner.
Customers rely on the HiGroTM incubated shaker to provide high-throughput and reproducible results. The most common application that the HiGro shaker is used for is E.coli growth in 96- and 384-well plates to produce high-copy plasmid DNA.
A high-throughput and low-waste viability assay for microbes
Nov 2, 2023 · GVA computes a sample’s viable cell count on the basis of the distribution of embedded colonies growing inside a pipette tip. GVA is compatible with Gram-positive and Gram-negative planktonic...
A 96-well-plate–based optical method for the quantitative and ...
Jul 29, 2010 · In this paper, we describe a fast and reliable protocol for using 96-well microtiter plates for the formation of Pseudomonas aeruginosa biofilms; the method is easily adaptable for...
A Guide to Bacterial Culture Identification And Results …
Apr 13, 2016 · Gram-positive bacteria that are associated with true infection include Staphylococcus aureus and Streptococcus pneumoniae. Repeat cultures should be drawn to ensure the resolution of bacteremia. To diagnose pneumonia, many clinicians order sputum cultures, endotracheal aspirates, and, less frequently, broncho alveolar lavage.