Isolation of primary mice splenocytes for in vitro research
Jan 1, 2020 · We isolated mouse splenocytes from Swiss albino mice by using a mechanical method and cultured them in medium with fetal bovine serum, 1% penicillin-streptomycin and 1% amphotericin as antibiotic ...
T cell proliferation assay -whole splenocytes or T cell isolation is ...
Jan 9, 2017 · So, for example, if you just mix CD90.1 CFSE labeled Tg whole splenocytes with whole unlabeled CD90.2 splenocytes supposed to play the role of APCs, then you can certainly do an accurate CFSE ...
Isolation of mouse splenocytes (A) Mouse was sacrificed
Isolation of mouse splenocytes (A) Mouse was sacrificed and immersed in 75% ethanol. (B) Exposure of the spleen. (C) Spleen in a sterile collection tube. (D) Place the spleen in a sterile dish ...
Stimulation of OVA-immunized splenocytes in vitro . Splenocytes …
Splenocytes collected from C57BL/6 mice intraperitoneally immunized with OVA were cultured for 72 h or 96 h with either 5 g/ml of free OVA, lipo-OVA, or MGlu-HPG-lipo-OVA.
What does "peptide pulsed and unpulsed syngeneic splenocytes" …
To do this, splenocytes were incubated with high concentration of CFSE and, then, antigenic peptide to achieve its binding with MHC class I molecule. Such pulsed splenocytes expressing complex MHC ...
Regulation of delta opioid receptor expression by anti-CD3-e, PMA, and ionomycin in murine splenocytes and T cells Ming D. Li, Kathy McAllen, and Burt M. Sharp
Culturing splenocyte and keeping them alive? - ResearchGate
Apr 3, 2017 · Splenocytes from a TCR-5/4E8-Tg mouse, a mB29b-TCR Tg mouse [51] and Balb/c WT mouse were cultured in 200 μl complete medium for 72h at 2x105 cells/well in the presence of 20 and 100 U/ml IL-2 or ...
What is your best splenocyte/lymphocyte restimulation protocol?
Then I plate 6 million splenocytes at a density of 1 million/mL in one well. The media I use is RPMI 1640 with 10% FBS, 1% P/S and 1% HEPES. I then add 2 μg/mL of anti-CD28 and 250U of IL-2 to ...
How can a mouse splenocyte be cultured? | ResearchGate
Then I plate 6 million splenocytes at a density of 1 million/mL in one well. The media I use is RPMI 1640 with 10% FBS, 1% P/S and 1% HEPES. I then add 2 μg/mL of anti-CD28 and 250U of IL-2 to ...
(PDF) Interaction of mouse splenocytes and macrophages with …
Dec 21, 2015 · We used splenocytes and naïve or interferon-γ-stimulated bone marrow-derived macrophages (BMDM) as responder populations. All tested bacterial strains induced phenotypic and cytokine responses ...