Lipid accumulation in Huh7 cells. Lipid accumulation was detected in ...
The HepG2 cells were treated with a mixture of palmitate-oleate to induce steatosis; after 24 h of treatment with the test materials, the intracellular lipid content was estimated using Oil...
Characterization of Huh7 cells after the induction of insulin ...
To normalize cell confluence, Oil Red O staining cell monolayers were stained with a crystal violet staining solution (0.5 g of crystal violet powder dissolved in 80 mL of distilled water and 20 mL of absolute methanol) by 5 min.
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Feb 2, 2004 · Detection of intracellular lipid droplets in HuH7 cells. (A) Human hepatocyte cell line HuH7, cultured in DMEM supplemented with 10% FBS, was fixed and stained with Oil red O and Mayer's hematoxylin. Intracellular lipid droplets were detected as red spheres in the cells. Nuclei are stained in blue.
Apigenin prevents oleic acid (OA)-induced lipid accumulation in …
Oil red O and Nile red staining showed that the intracellular levels of lipids in Huh7 cells were increased in response to OA challenge ( Fig. 1A and B). Importantly, the OA-induced...
Lipid accumulation in HepG2 and Huh7 cells treated with
Oil Red O staining and BODIPY staining were performed to determine lipid accumulation in Human umbilical vein endothelial cells (HUVECs). Foam cell formation and monocyte recruitment were...
miR-122 promotes hepatic lipogenesis via inhibiting the …
Jun 13, 2019 · As expected, Oil Red O staining showed that FFA treatment remarkably induced lipid deposition inside cells (Fig. 2a). Moreover, FFA challenge robustly elevated TG secretion from HepG2 and Huh-7 cells (Fig. 2 b).
Reduction in Insulin Mediated ERK Phosphorylation by Palmitate …
Sep 2, 2022 · In this study, we investigated the effects of ER stress on insulin resistance and ERK phosphorylation in Huh-7 cells and evaluated how oleate prevents palmitate-mediated ER stress.
Schaftoside alleviates HFD-induced hepatic lipid accumulation …
Jun 12, 2020 · In vitro, the intracellular triglyceride (TG) level and Oil Red O staining were used to evaluate the lipid removal ability of SS in Huh-7 cells or FXR knockout mouse primary hepatocytes (MPHs) induced by oleic acid (OA). Moreover, FXR/sterol regulatory element-binding protein 1 (SREBP1) mRNA and protein expression levels were detected.
Naringenin Attenuates Non-Alcoholic Fatty Liver Disease by ... - PubMed
Jun 7, 2021 · L02 and Huh-7 cells were induced by sodium oleate to establish a NAFLD cell model. The effects of NAR on lipid accumulation were detected by oil red O staining. The glucose uptake and ATP content of 3T3-L1 adipocytes and C2C12 myotubes were measured.
Remove Oil Red O solution and immediately add ddH2O. Wash the cells 4 times with ddH2O. Acquire images under the microscope for analysis. Remove all the water and let dry. Elute Oil Red O dye by adding 1 ml of 100% isopropanol and incubate for 10 min with gently shaking.