Dec 12, 2021 · In this study, we proposed a strategy to select functional variable transcripts from murine hybridoma clones by using two sets of specific primers covering most of the mouse immunoglobulin genes to selectively amplify diferent V or …
Efficient amplification and direct sequencing of mouse variable …
Jun 11, 1999 · The antisense primer RevCκSalI (5′-CGACTA GTCGAC TGGTGGGAAGATGGATACAG-3′) contains the reverse complement of codons 113–120 of kappa light chain constant region.
Two databases of 491 mouse heavy chain and 356 mouse light chain signal sequences, expanded from the Kabat database ([32], http://immuno.bme.nwu.edu/), were used to design the PCR primers.
Universal PCR amplification of mouse immunoglobulin gene …
Jan 13, 2000 · A single isotype-specific primer in the first domain of the constant region was designed for each heavy isotype gene and one constant region primer for kappa light chains (Table 2). The lambda light chain was ignored in primer design as most (95%) expressed mouse Ig genes are kappa isotype (Honjo and Alt, 1995); in fact, we encountered no ...
Analysis of heavy and light chain sequences of conventional …
Mar 1, 2014 · Here we study the sequence diversity of functional variable and constant regions observed in 57 conventional heavy, 18 kappa and 35 lambda light chains of C. dromedarius and Camelus bactrianus. We detail sequences of the full kappa and lambda light chain, variable and CH1 region for IgG1a and IgG1b and the CH2 and CH3 region for IgG1a.
The mouse immunoglobulin kappa light-chain genes are located …
The murine immunoglobulin kappa (kappa) light-chain multigene family includes the constant region (C kappa), joining-region genes, and approximately 30 kappa-variable (V kappa) region families. The entire region occupies an estimated 1,000 to 3,000 ...
IgG, Library Primer Set, Mouse, BioGenomics™ - usbio.net
Moreover, the randomized combination of cloned heavy and light chain immunoglobulin gene fragments allowed the construction of mouse antibody libraries. These libraries enable the isolation of specific antibodies against particular antigens by phage display techniques.
Design and characterization of a plasmid vector system
Sep 6, 2008 · Sequence-specific primers are used to clone antibody constant domains, allowing for the creation of antibodies of multiple isotypes from a variety of lineages. A high-efficiency leader sequence has been inserted into the start of each gene to improve secretion.
Insights into the regulation of immunoglobulin light chain gene ...
Kde-mediated rearrangements were analysed using a set of seven framework 1 family-specific 5′ primers (VκI to VκVII, Fig. 1a, primer A) and a 3′ primer annealing to Kde (Fig. 1, primer B). 30 In order to identify the Vκ family employed, separate PCR reactions for each 5′ primer were used.
An extended primer set for PCR amplification of murine kappa …
The design and successful usage of an extended primer set for the PCR amplification of murine variable kappa light chain sequences from mouse hybridomas are described. Since some of these primer pairs also amplify the endogenous SP2/0 aberrant light chain sequence, strategies to distinguish the irre …