Isolation and Culture of Human Dermal Fibroblasts - PubMed
Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin.
Primary Dermal Fibroblast; Normal, Human, Adult (HDFa)
Primary Dermal Fibroblast; Normal, Human, Adult (HDFa) is a skin cell line with research applications in responding to pathogens, skin aging, wound healing, gene delivery, and skin diseases, including scleroderma.
Dermal fibroblast - Wikipedia
Dermal fibroblasts are responsible for creating the ECM which organizes the stratified squamous epithelial cells of the epidermis into a unified tissue. Furthermore, dermal fibroblasts create long fibrous bands of connective tissue which anchor the skin to the fascia of the body.
Dermal Fibroblasts as the Main Target for Skin Anti-Age …
Analyzing these data, the authors proposed the concept of skin anti-age therapy that is based on the correction of age-related skin changes by stimulating regenerative processes at the molecular and cellular level. The main target of the skin anti-age therapy is dermal fibroblasts (DFs).
Single-cell transcriptomes of the human skin reveal age ... - Nature
Apr 23, 2020 · Our work thus provides evidence for a functional specialization of human dermal fibroblasts and identifies the partial loss of cellular identity as an important age-related change in the...
The Role of Fibroblasts in Skin Homeostasis and Repair - PMC
Light microscopy of human dermal fibroblasts at low (A) and high (B) cumulative population doubling. When cells reach replicative senescence, fibroblasts become larger and accumulate cytoplasmic vacuoles (B). Images are from authors’ laboratory.
Cross-tissue human fibroblast atlas reveals myofibroblast …
Sep 19, 2024 · Fibroblasts, known for their functional diversity, play crucial roles in inflammation and cancer. In this study, we conduct comprehensive single-cell RNA sequencing analyses on fibroblast cells from 517 human samples, spanning 11 …
Human Dermal Fibroblast Subpopulations Are Conserved across …
Jul 1, 2021 · We have found three major human adult dermal fibroblast types and 10 subtypes that account for 92.5% of the fibroblasts whose transcriptomics have been analyzed by scRNAseq to date in four different studies.
The effect of aging in primary human dermal fibroblasts
Jul 3, 2019 · In this study, we analyzed primary human dermal fibroblasts in three different in-vitro aging models—UVB irradiation and accelerated proliferation of human dermal fibroblasts from young donors as well as from elderly donors—for the gene expression of COL1A1, COL1A2, COL3A1, COL4A1, COL7A1, MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP12 ...
Fibroblast origin shapes tissue homeostasis, epidermal …
Feb 27, 2019 · Normal human dermal fibroblasts (NHDF) from the breast skin of women aged 20–30 years (“adult”) or 60–70 years (“aged”), as well as NHDFs from the foreskin of boys <10 years old (“juvenile”), were cultured in monolayers or within the dermal compartment of …