The Cre-Lox system is widely used to manipulate gene expression in model organisms such as mice in order to study human diseases and drug development. [3] For example, using the Cre-Lox system, researchers are able to study oncogenes and tumor suppressor genes and their role in the development and progression of cancer in mouse models.

The Cre/ lox system is one of the most powerful and versatile tools developed for mouse genetics. It gives mouse researchers sophisticated control over the location and timing of gene expression.

Two of the most exciting and versatile genetic tools designed in the last 30 years are the Cre-lox and FLP-FRT technologies. Both allow the location and timing of gene expression to be closely regulated. This article briefly outlines how the two systems work and can be used in …

In principle, one mouse must have a tissue-specific driven cre gene and another mouse have loxP flanked (floxed) alleles of interest gene Y. Expression of Cre recombinase excises floxed loci and inactivates the gene Y. Cre- loxP system is a widely used powerful technology for …

The tamoxifen-inducible Cre strain, B6.Cg-Tg (CAG-cre/Esr1)5Amc/J (004682), is used to conditionally delete floxed sequences throughout the entire mouse because its promoter (beta actin) should have ubiquitous expression.

Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus.

The Cre- loxP system remains the gold standard method for conditional gene regulation in mice, but it can be costly and time consuming. So, over the last few years, researchers wondered …

The Cre-lox recombination method orchestrates remarkable genetic manipulations that remain a gold standard for transgenic mice.

Oct 20, 2020 · The Cre-Lox system enables gene expression with exceptional spatial (expresses a gene in specific cells, tissues, or entire organisms) and temporal (at specific time points through the application of the Cre-Lox system) control.

Most investigators breed a new Cre-recombinase-expressing (Cre-driver) line of mice with a Reporter line of mice in which expression of the Reporter is activated by deleting a ‘lox-stop-lox’ sequence to test for the specificity of the Cre-induced recombination in the offspring.