pCMV-Gaussia Luc Sequence and Map - SnapGene
Control vector for constitutive high-level expression of secreted Gaussia luciferase under control of the CMV promoter. Sequence Author: Thermo Fisher (Pierce) Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets. Efficient cleavage requires at least two copies of the PaqCI recognition sequence.
pMCS-Gaussia Luc Sequence and Map - SnapGene
Secreted Gaussia luciferase vector for cloning a transcriptional regulatory element. Sequence Author: Thermo Fisher (Pierce) Explore Over 2.7k Plasmids: Luciferase Vectors | More Plasmid Sets. Efficient cleavage requires at least two copies of the PaqCI recognition sequence. Sticky ends from different PaqCI sites may not be compatible.
Application of Gaussia luciferase in bicistronic and non …
Jul 9, 2014 · We describe three basic mechanisms by which GLUC can be released from cells: first, classical secretion by virtue of the N-terminal signal peptide; second, internal signal peptide-mediated secretion and third, non-conventional secretion in the absence of …
Advanced Bioluminescence Reporter with Engineered Gaussia Luciferase ...
Gaussia luciferase (GLuc) is the preeminent secreted luciferase widely used in cell-based reporter assays. By employing sequence-guided mutagenesis informed by alignments of diverse copepod luciferase sequences, we identified key amino acids that …
pCMV-GLuc 2 Sequence and Map - SnapGene
Control vector with a strong constitutive CMV promoter for expressing secreted Gaussia luciferase in mammalian cells.
7D2O: Solution structure of Gaussia Luciferase by NMR - RCSB PDB
Sep 17, 2020 · Gaussia luciferase (GLuc) is a small luciferase (18.2 kDa; 168 residues) and is thus attracting much attention as a reporter protein, but the lack of structural information is hampering further application. Here, we report the first solution structure of a fully active, recombinant GLuc determined by heteronuclear multidimensional NMR.
Gaussia princeps luciferase - Molecular Imaging and Contrast …
Jan 31, 2008 · Gaussia princeps, a marine copepod, naturally secretes a luciferase (GLuc) that was cloned, expressed in Escherichia coli, and used as a detection agent in a DNA hybridization assay (13).
Bioluminescent and structural features of native folded Gaussia luciferase
Jun 1, 2018 · The GpLuc coding sequence with native signal peptide was amplified using pCMV-GLuc template (NanoLight Technology) with humanized Gaussia luciferase and specific primers: forward 5′-ACAGGTACCATGGGAGTCAAAGTTCTG-3′ with KpnI site (underlined) and two overlapping reverse primers: first 5′-TGATGATGACCTTGAAAGTACAAGTTCTCGTCACCACCGGCCCCC-3 ...
Superluminescent variants of Gaussia luciferase in living animal …
In this study, we have engineered two novel luciferases, BeM and SuM, by incorporating known amino acid mutations that have demonstrated the ability to stabilize, enhance, or shift the wavelength of Gluc-catalyzed luminescence towards the red spectrum.
Membrane-bound Gaussia luciferase as a tool to track shedding …
Nov 22, 2019 · A characterization of membrane-bound Gaussia luciferase (mbGluc) revealed that its signal was detected also in a form smaller than common EVs (<70 nm).