Optimal conditions for fluorescence of proteins of the actin and tubulin cytoskeleton are based on: preserving cell structure; properties of individual cytoskeletal proteins and any antibodies to be used; background fluorescence.
Staining Actin and Tubulin – from WB to Live Cell Imaging
May 6, 2015 · Actin and Tubulin, as the major cytoskeleton structures, are crucial components of a plethora of processes in cell biology. Both are very much involved in stabilizing the cell shape, and especially in cell movements (e.g. cell migration) and intracellular movements and …
Actin in Action: Imaging Approaches to Study Cytoskeleton …
In this review we provide an introduction to various imaging modalities used to study focal adhesions and the actin cytoskeleton, and using specific examples we highlight a number of recent studies in animal cells that have advanced our knowledge of cytoskeletal behavior.
Actin staining techniques in fixed and living cells
Feb 3, 2015 · Spirochrome recently released unique fluorescent stains for monitoring Actin (and Tubulin) easily in living cells. These fluorescent stains (SiR-Actin and SiR-Tubulin) are cell permeable compounds which stain F-actin (and microtubules, respectively) in living cells.
Labeling protocol for Tubulin, Actin and DNA in living specimens
Staining of intracellular targets using abberior LIVE tubulin, actin, and DNA labels. The procedure described below has been successfully tested with our abberior LIVE labels for direct labeling in several adherent mammalian cultured cell lines and Drosophila melanogaster dissected organs. This procedure has yielded consistent results in most ...
Actin Staining Techniques - Actin staining protocols, Actin stain ...
Actin stains and probes are useful biological reagents for detecting and visualizing actin in cells and tissues, actin staining with Acti-stain 488 phalloidin is the best option for bright and stable fluorescence imaging, fluorescent actin, actin antibody, stem cell stain, stem cell marker, sarcomere stain, muscle cell stain, fluorescent actin.
Fluorogenic probes for live-cell imaging of the cytoskeleton
May 25, 2014 · We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells.
Detection of Actin, Intermediate Filaments, and Tubulin
Fluorescent and biotinylated phallotoxins allow labeling of F-actin in fixed cells and can be used interchangeably in most applications. They stain F-actin at nanomolar concentrations and are water soluble, providing convenient probes for labeling, identifying, and quantitating F‑actin.
2 new Actin and Tubulin live cell imaging stains - Tebubio's blog
Oct 30, 2014 · Cell biologists at tebu-bio have selected Spirochrome’s unique fluorescent stains for monitoring Actin and Tubulin easily in living cells. These fluorescent stains (SiR-Actin and SiR-Tubulin) are cell permeable compounds which stain microtubules and F …
Probes for Tubulin and Other Cytoskeletal Proteins—Section 11.2
Label tubulin in live or fixed cells using Molecular Probes fluorescent reagents, including paclitaxel conjugates, antibodies, and tubulin-targeted GFP and RFP.