This is a useful study that adds new data to how different DAG pools influence cellular signaling, and dissects how the enzyme Dip2 modulates the minor lipid signaling DAG pool, which is distinct from ...

This manuscript provides valuable information about the genesis of CPSF6 condensates due to HIV-1 infection. However, the evidence is incomplete as it is missing more functional assays. Furthermore, ...

Autophagy was quantified by western blotting of LC3, transmission electron microscopy analyses and confocal microscopy scanning of mRFP-GFP-LC3 reporter construct. Luciferase reporter assays were ...

RT-qPCR assay was performed precisely following the protocol in a previous study (25). The sequence of primers used in the present study were presented in Supplementary Table 2. Western blotting was ...

This protocol describes the design of a study on sexual well ... so the personal information of participants can be protected. HIV-1 Western Blot Bio-Rad assay (Bio-Rad Laboratories, Redmond, ...

Cells were then washed once with PBS and lysed in lysis buffer for Western blot analysis. For chronic insulin treatment ... The protein content was determined using Pierce Micro BCA protein assay ...

CCD cameras usually have larger pixels than CMOS cameras. The benefit of larger pixels is more surface area to gather photons ...

Quantum Dot Corporation's (QDC) new western blot detection kits harness the high brightness, extreme stability and multiple colors of the Qdot Secondary Antibody Conjugates to quantitatively ...

The assay was reworked as a consequence ... share common false reactivity should not be used in combination. A western blot is typically the final step in a diagnostic algorithm, because if ...

Methods: Using quantitative reverse transcription-PCR (RT-PCR), immunofluorescence, immunohistochemistry, western blotting, as well as gain- and loss-of-function studies and functional assays, the ...