Owing to extensive accumulation of primer-dimers when no template control is used during one-step RT-PCR, we introduced a two-step protocol to eliminate this problem. This study further ...

But what is real time RT–PCR? How does it work? How is it different from PCR? And what does this have to do with nuclear technology? Here’s a handy overview of the technique, how it works and a few ...

or real-time RT–PCR. Fast and accurate, real-time RT–PCR has been critical in detecting, tracking and studying COVID-19. But what is it, and how does it work? Let us explain.