School of Cardiovascular and Metabolic Medicine & Sciences, King’s College London, London WC2R 2LS, U.K. Faculty of Medicine, Department of Biomedical Sciences, Masaryk University, 62500 Brno, Czech ...
Cells were then washed once with PBS and lysed in lysis buffer for Western blot analysis. For chronic insulin treatment experiments, IHH cells were treated for 5 days in regular culture medium with 10 ...
Department of Pharmaceutics, School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery, Ministry of Education, State Key Laboratory of Medical Neurobiology and MOE Frontiers Center ...
were used to generate libraries through copper-catalyzed azide-alkyne cycloaddition synthesis that were screened with the in-cell western assay (phosphorylated JNK detection) using U2O2 cells ...