eLife16d
B cell expression of the enzyme PexRAP, an intermediary in ether lipid biosynthesis, promotes antibody responses and germinal center size
This study provides useful insights into the ways in which germinal center B cell metabolism, particularly lipid metabolism, affects cellular responses. The authors use sophisticated mouse models to ...
C&EN17d
Porous Silicon on Paper: A Platform for Quantitative Rapid Diagnostic Tests
Hydrofluoric acid (HF, 48–51% solution in DI water), 3-aminopropyltriethoxysilane (APTES, 99%), EZ-Link sulfo-NHS-biotin, streptavidin, methanol, ethanol (200 proof), phosphate-buffered saline (PBS), ...
Frontiers19d
Rab10-associated tubulation as an early marker for biogenesis of the assembly compartment in cytomegalovirus-infected cells
Pericentriolar (pc) and perinuclear (pn) accumulation of Rab10 fluorescence signal in MCMV-infected cells ... signal (t x hpi)/normalized experimental signal (t 0 hpi). 2.12 Biotin labelling of NIH ...
Frontiers24d
Productive biosensing techniques empowered by all-dielectric metasurfaces
Two major types of metasurface biosensors have been reported: one is based on resonant-wavelength shift and the other is specialized for fluorescence (FL) detection. The all-dielectric metasurfaces ...
C&EN25d
Specific Protein Quantification by Radioimmuno-Dot-Blot Assay for Complex Mixture Samples Utilizing Strep-Tag and Tritium-Labeled Strep-Tactin
Creative Commons (CC): This is a Creative Commons license. Attribution (BY): Credit must be given to the creator. Accurately quantifying specific proteins from complex mixtures like cell lysates, for ...
The EMBO Journal28d
The proximity-based protein interactome and regulatory logics of the transcription factor p65 NF-κB/RELA
Intracellular biotinylation was induced by the addition of 50 µM biotin for further 60 min during which time half of the samples were additionally treated with IL-1α (10 ng/ml). Cytosolic and nuclear ...