The two sgRNAs were then cloned into linearized pX330 using T4 DNA ligation (catalog No. N103-01, Vazyme). To generate the donor plasmid, the homologous arms upstream and downstream of the Cas9 ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. To address a wide range of genetic diseases, ...

Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. Oncolytic adenoviruses were designed to reach ...

Texas A&M Drug Discovery Center, Department of Chemistry, Texas A&M University, College Station, Texas 77843, United States Institute of Biosciences and Technology and Department of Translational ...

RNA extraction, reverse transcription using InvAR17 primer, cDNA clean-up using silane beads (Thermofisher), second adaptor ligation (InvRand3Tr3) and cDNA purification steps were performed as ...

This valuable work examines the role of the oxidoreductase MftG in Mycolicibacerium smegmatis alcohol metabolism. A solid set of data is presented to show that, in the context of ethanol metabolism, ...

DNA Sequencing is the process of reading the nucleotides present in DNA: determining the precise order of nucleotides within a DNA molecule. DNA-Seq generally refers to any NGS method or technology ...

Slips are prepared by your employer, payer, administrator or financial institution. You should receive most of your slips (including your T4, T4A, and T5 slips) and receipts by the end of February.

Internet filing is available starting January 13, 2025. Our Internet filing applications are convenient, free and secure. Where possible, you should file returns electronically to avoid the delays ...