This is a useful study that adds new data to how different DAG pools influence cellular signaling, and dissects how the enzyme Dip2 modulates the minor lipid signaling DAG pool, which is distinct from ...
This is a useful study that seeks to address the role of the TET family of DNA demethylation enzymes in pancreatic beta cell senescence in the context of type 2 diabetes (T2DM). Although the concepts ...
Herein, we describe the development of a specific protein quantification method that combines a solid-state dot blot approach with radiolabel detection ... The fixing step was not part of the original ...
2.3 Isolation of smooth ... samples (ΔΔCT method). For Western blot experiments total cell lysates were isolated from control, calcified and gene silenced HAoSMC cell lines. The samples were ...
For western blot analysis, 25 μg of total protein (measured by the Lowry method ... sections were treated equally (same duration of fixation, immunohistochemical protocol, and antibody dilution), ...
For detection, Envision antirabbit IgG (Dako) was used according to the manufacturer’s protocol. For 5’-bromo-2 ... and supernatant was collected. For western blot analysis, 60 µg protein was ...
Cells were then washed once with PBS and lysed in lysis buffer for Western blot ... protocols below were followed: To measure oleate storage in intracellular lipids, cells were washed and collected in ...
Call it the science of small, but a trio of University of Florida chemical engineers have developed a lab-on-a-chip process ...
Purification removes contaminants from DNA samples, ensuring the integrity of the genetic material and providing more ...
We investigated the association between proteins and self-reported loneliness and social isolation. We discovered that the proteins found to be significantly associated with loneliness and social ...