Cells were then washed once with PBS and lysed in lysis buffer for Western blot analysis ... Cells were lyzed in a buffer containing 10 mM NaF, 0.25 M EGTA, 25 mM NaCl, 1% IGEPAL, 1 mM sodium ...
Cells were lysed in RIPA lysis buffer containing 50 mM Tris–HCl, 150 mM NaCl, 0.5% sodium deoxycholate, 1.0% NP-40, 0.1 mM PMSF, 0.5 mg/mL leupeptin, 1.0 μM pepstatin, and 0.3 μM aprotinin. Total ...
“The investment scenario is brighter, as corporations step into 2025 with robust balance sheets and high profitability," said Malhotra. The Financial Stability Report (FSR) noted that the 6.6% ...
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0–3-hour embryos were lysed in Co-IP Lysis Buffer (20mM Tris pH 8.0, 137mM NaCl, 1% IGEPAL, 2mM EDTA, 1X PIC) using a Dounce homogenizer, and centrifuged at 21,000 g for 30 minutes. 3 mg of total ...
Department of Molecular, Cell and Systems Biology, University of California Riverside, Riverside, USA Stowers Institute for Medical Research, Kansas City, USA ...
Protocol Buffers (a.k.a., protobuf) are Google's language-neutral, platform-neutral, extensible mechanism for serializing structured data. You can learn more about it ...