After 4 days, we performed tartrate-resistant acid phosphatase (TRAP) staining and determined the amount of OCs ... Illumina), following the manufacturer’s protocol. The libraries were sequenced for ...
T4 DNA ligase, T4 DNA polymerase, Klenow fragment, T4 polynucleotide kinase, shrimp alkaline phosphatase ... carried out using the protocol of Price and Naumann (27). Equal volumes of matrix ...
A uric acid test measures the amount of a waste product called uric acid (urate) in your blood or urine. Uric acid is a byproduct of the breakdown of chemicals called purines found in foods and drinks ...
The experimental protocol was approved by Animal Welfare Ethics Committee of Shanghai Sixth People’s Hospital (NO: DWLL2020-0550). C2C12 myoblasts (1 × 10 4 cells/well) in 96-well plates were ...
BM mononuclear cells were isolated by Ficoll-Paque Premium (GE Healthcare) density-gradient centrifugation according to the manufacturer's protocol. For apoptosis ... activated Chk1 phosphorylates the ...
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