g., for microarray experiments Enzymatic assays (e.g., peroxidase, alkaline phosphatase, β-galactosidase) Optical density of cells (OD 600) Both measurement and calculation of results are ...

1| Pellet ∼10 8 cells at 300g at 4 °C for 10 min in a centrifuge. The nuclei should be kept cold throughout the procedure. Manipulations and transport to the centrifuge should be done on ice. 2 ...