She was using western blotting to study large ribosomal proteins, employing a protocol with two overnight steps. “Finally, I had that blot, and went to image it, but there was nothing there.

Western blotting forms the bedrock of many protein studies, with the technique cited in 9% of all protein related papers. The technique had its 40th birthday in 2019, an event that was widely ...

Western blot analysis was performed on the lysate of the other half of each kidney. Protein extraction was performed using an ActiveMotif extraction kit according to the manufacture's protocol ...