Because factors such as sample quality, integrity, and purity influence experimental outcomes, researchers often rely on probe-based qPCR to increase the technology’s specificity and sensitivity.
By contrast, probe-based assays ensure high specificity through the combination of gene-specific primers and the qPCR probe. But the probe-based assay format is less flexible, requiring the design ...
MGB ValuMix assays for qPCR gene expression offer the convenience of receiving a customized, dual-labeled MGB probe and primer pair in a single tube. The proportions are precisely delivered ...
You can supply the specifications, including the probe-to-primer ratio, for gene expression and qPCR assays, and LGC sends you a unique, dual-labeled LNA probe and primer pair in a single tube.
For experiments that aim to detect or quantify multiple targets, two or more probe-based qPCR assays can be combined in a single reaction well. However, developing a multiplex qPCR experiment is more ...
ABsolute Fast QPCR Master Mixes are compatible with any sequence-specific probe. In addition to accelerating QPCR analysis of DNA, the master mix can also be used for quantitative reverse ...