ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described ... (predominantly SYBR Green) and probe-based (for example, hydrolysis probes).

BHQplus Probes form more stable probe-target duplexes, enabling shorter oligonucleotide designs. Shorter oligos offer high specificity and better detection of difficult targets, such as AT-rich ...

Molecular Beacons do not rely on probe hydrolysis from the 5' exonuclease activity of Taq polymerase to generate fluorescence, therefore, a post-PCR melt curve is possible. Just like standard dual ...

Qualitative end-point RT-PCR can be used for minimal residual ... chemistries (e.g., DNA binding dyes, TaqMan® hydrolysis probes [Roche], Lightcycler® dual hybridization probes [Roche ...

and 48 primer/probes (each a combination of forward and reverse PCR primers specific to the gene of interest and a fluorescent resonance energy transfer (FRET) hydrolysis probe located between the ...