This causes line emission spectra to be produced, as shown below. This is not a continuous spectrum as only light of specific frequencies and specific colours are produced. Different types of ...

Previous analyses have shown that eYFP yields detectable emission in the 400–500 nm range under particular conditions 5. Our results extend these studies by showing that photobleaching of eYFP ...

A second laser, called the STED laser, is used to create a donut-shaped intensity profile that overlaps with the excitation laser. The STED laser has a wavelength that matches the emission spectrum of ...