Accordingly, the total loss of DNA ligase function is lethal. DNA ligases catalyze the joining of a 5’-phosphate-terminated strand to a 3’-hydroxyl-terminated strand. Ligation depends on magnesium and ...
They function in vivo to repair a break in E ... domain belongs to the covalent nucleotidyltransferase enzyme superfamily that includes classic RNA/DNA ligases and mRNA capping enzymes. We’ve shown ...
After two fragments with complementary sticky ends are joined, the DNA backbone may be covalently sealed using another enzyme called DNA ligase.
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