Thermo Fisher researchers took a standard human carcinoma line (A-431) and used a western blot to get a baseline for the binding signal. They then used CRISPR-Cas9 to eliminate the target gene and ...
Western blot analysis showed a single band at the expected size of 66 kDa. The signal was absent in lysates of cells in which OPTN had been knocked out using CRISPR-Cas9, confirming the ...
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