Reengineered streptavidin mutants bind biotin-4-fluorescein with extremely tight yet reversible binding affinity. The terrifically tight noncovalent interaction between biotin and streptavidin has ...

The phosphorylation of the biotin-tagged generic ... (the time-resolved fluorescence resonance energy transfer (HTRF) donor), and a streptavidin-XL665 (the HTRF acceptor). Figure 1: All assays ...